| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Podoliak, Kaleb A (10)
|
NIKRKN |
46.14 |
45.65 |
-0.49 |
| 2 |
Arron, Cooper J (10)
|
NIUNAC |
46.22 |
47.68 |
1.46 |
| 3 |
Smith, Liam B (10)
|
NIGGST |
48.04 |
48.36 |
0.32 |
| 4 |
Gorman, Ryan P (10)
|
NIWAAC |
50.41 |
48.48 |
-1.93 |
| 5 |
Anderson, Koen T (9)
|
NIISWM |
53.13 |
53.16 |
0.03 |
| 6 |
Losinger, Shawn B (10)
|
NIUNAC |
55.24 |
55.48 |
0.24 |
| 7 |
Ankam, Ronel P (10)
|
NIWAAC |
58.15 |
56.44 |
-1.71 |
| 8 |
Reimsnyder, Marvin W (10)
|
NIHAWK |
54.11 |
57.60 |
3.49 |
| 9 |
Worob, Jack H (10)
|
NIISWM |
51.34 |
57.63 |
6.29 |
| 10 |
Wu, Winston X (10)
|
NIHAWK |
59.27 |
58.17 |
-1.10 |
| 11 |
Gold-Rogers, Griffin C (9)
|
NIISWM |
59.52 |
58.20 |
-1.32 |
| 12 |
Matt, Lucas (9)
|
NIVEST |
1:04.54 |
1:00.49 |
-4.05 |
| 13 |
Mahon, Benjamin G (9)
|
NIHAWK |
1:04.52 |
1:04.82 |
0.30 |
| 14 |
Hayden, Jack D (9)
|
NIGGST |
|
1:07.53 |
- |
| 15 |
Walters, Alex J (9)
|
NIGGST |
1:13.88 |
1:07.77 |
-6.11 |
| 16 |
Peters, Nathan L (9)
|
NIUNAC |
|
1:09.79 |
- |
|
Skorusa, Kaleb M (10)
|
NIGGST |
|
DQ |
- |
|
Dean, Ryan C (10)
|
NIGGST |
|
DQ |
- |